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January 2013

It's still only January but Nature Protocols February 2013 issue has been published, including this month's featured protocol:

The mouse excisional wound splinting model, including applications for stem cell transplantation

For a limited time, read the full text article for free at Nature Protocols.


December 2012

This month we decided to take a closer look at the rapidly evolving field of epigenetics - the study of heritable, regulatory changes in gene expression that are modulated independently of changes to the underlying DNA sequence. We came across Epigenie - a great information resource for all things related to epigenetics.

To learn more about this exciting field, take a look one at Epigenie's recent posts - 5hmC timeline - which chronicles the evolution of 5hmC analysis methods.

Then check out some of the recently cited published articles:

• The anti-CMS technique for genome-wide mapping of 5-hydroxymethylcytosine

• The GLIB technique for genome-wide mapping of 5-hydroxymethylcytosine

• Rapid and quantitative method of allele-specific DNA methylation analysis

And vendor solutions cataloged at MySharedProtocols:

• Active Motif's MethylCollector™ Kit for efficient isolation of methylated CpG islands

• Epigentek's EpiQuick Methylated DNA Immunoprecipitation Kit for enrichment and isolation of methylated DNA

• Zymo Research's OneStep qMethyl™ Kit for the detection of locus-specific DNA methylation

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